Tobacco Potentially as antiseptic

Teams of students Department of Biochemistry, Faculty of Mathematics and Natural Sciences (Natural Sciences), Bogor Agricultural University, through a study found the use of tobacco plants nonrokok innovation. The research team headed by Riska Ayu Purnamasari managed to find the potential of tobacco as a killer germ extract or antiseptic.

"Our research is proving the antimicrobial extracts of tobacco to be applied as an antiseptic. It is as an effort to find common ground that can benefit any health-related elements of society and culture grow tobacco," he said.

According to Riska, tobacco extract compounds that are identified as testing antimicrobials against several strains of microbes. Several studies have demonstrated the antimicrobial properties of tobacco extracts against E coli and S aureus and P aeruginosa.

"Tobacco extract has potential in inhibiting the growth of bacteria E coli and S aureus. The results indicate that extracts the ethanol extraction of essential oil of tobacco is the most widely extracts obtained during the extraction process carried out and the most effective to inhibit bacterial growth," he said.

He explained that the minimum growth inhibitory concentration of essential oil extracts of 10 percent demonstrates the inhibitory essential oil of tobacco is quite large, and results in a topical test showed significant differences between the situation before and after smeared.

Tobacco extract antimicrobial activity assay performed using compounds extracted, including essential oils, alkaloid compounds, and polyphenol compounds. Each extract is injected into the medium Nutrient Agar (NA) containing the test bacteria E coli and S aureus.

Clear zone formed around the well hole diffusion is the response of active antimicrobial extract in inhibiting bacterial growth. The result is known that the dilution of volatile as much as 10 percent have a fairly good inhibitory power according to the method of Davis-Stout.

Then, to see the potential of tobacco leaf extract as an antiseptic antiseptic potency test is performed through organoleptic tests with topical methods. Topical method performed on the five panelists. Panelists are dry palms smeared on Petri dishes containing PCA medium. Panelists then wash hands using extracts of tobacco and 70 percent ethanol as a positive control.